Journal: The Journal of Biological Chemistry

Article Title: BMI1 represses G-quadruplex DNA formation to maintain genomic stability during replication

doi: 10.1016/j.jbc.2026.111172

Figure Lengend Snippet: BMI1 knockdown in HDFs induces laminopathy . A , immunofluorescence analysis of HDFs at 24 h post-transfection with shScramble or shBMI1 plasmids. Scale bar: 10 μm. Deformation of the nuclear envelope, as labeled with Lamin A/C, was more frequent in shBMI1 HDFs ( arrows ). The bottom panel is a high magnification of cells with nuclear envelope deformation. Results are the sum of three experiments with 50 cells/experiment. Statistical differences were analyzed using an unpaired t test with two tails. ∗∗ p -value = 0.0092. All values are mean ± S.D. B , Western blot analysis of HDFs at 24 h post-transfection with shScramble or shBMI1 plasmids. Note BMI1 reduction in shBMI1 cells. Dashed black arrows indicate the position where the bands were cut from the original blots. The protein ladder indicates the molecular weight (MW) relative to the revealed bands. C , real-time PCR analysis of HDFs at 24 h post-transfection with shScramble or shBMI1 plasmids. While BMI1 expression was reduced and P16INKA was increased in shBMI1 cells, progerin levels were unaffected. Results are the sum of three experiments, with three replicates/experiment. Statistical differences were analyzed using an unpaired t test with two tails. ∗ p -value = 0.038, and ∗∗ p -value = 0.0043. All values are mean ± S.D.

Article Snippet: Real-time PCR was carried in triplicates using Platinum SYBRGreen Supermix (Invitrogen) and Real-time PCR apparatus (ABI prism 7002).

Techniques: Knockdown, Immunofluorescence, Transfection, Labeling, Western Blot, Molecular Weight, Real-time Polymerase Chain Reaction, Expressing